Enantioselective HPLC analysis and biodegradation of atenolol, metoprolol and fluoxetine

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Ribeiro, AR (Autor) Outra; Carlos Magalhaes Afonso (Autor) FFUP; Paula M L Castro (Autor) Outra; M E Tiritan (Autor) FFUP

Revista

Título: Environmental Chemistry Letters

Vol. 11

Páginas: 83-90

ISSN: 1610-3653

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 41 Citações

Scopus - 42 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia do ambiente

Outras Informações

ID Authenticus: P-002-056

DOI: 10.1007/s10311-012-0383-1

Abstract (EN): The accurate quantification of enantiomers is crucial for assessing the biodegradation of chiral pharmaceuticals in the environment. Methods to quantify enantiomers in environmental matrices are scarce. Here, we used an enantioselective method, high-performance liquid chromatography with fluorescence detection (HPLC-FD), to analyze two beta-blockers, metoprolol and atenolol, and the antidepressant fluoxetine in an activated sludge consortium from a wastewater treatment plant. The vancomycin-based chiral stationary phase was used under polar ionic mode to achieve the enantioseparation of target chiral pharmaceuticals in a single chromatographic run. The method was successfully validated over a concentration range of 20-800 ng/mL for each enantiomer of both beta-blockers and of 50-800 ng/mL for fluoxetine enantiomers. The limits of detection were between 5 and 20 ng/mL and the limits of quantification were between 20 and 50 ng/mL, for all enantiomers. The intra- and inter-batch precision was lower than 5.66 and 8.37 %, respectively. Accuracy values were between 103.03 and 117.92 %, and recovery rates were in the range of 88.48-116.62 %. Furthermore, the enantioselective biodegradation of atenolol, metoprolol and fluoxetine was followed during 15 days. The (S)-enantiomeric form of metoprolol was degraded at higher extents, whereas the degradation of atenolol and fluoxetine did not show enantioselectivity under the applied conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8