Lactoferrin-Derived Antimicrobial Peptide Induces a Micellar Cubic Phase in a Model Membrane System

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Margarida Bastos (Autor) FCUP; Tania Silva (Autor) Outra; Vitor Teixeira (Autor) Outra; Kamran Nazmi (Autor) Outra; Jan G M Bolscher (Autor) Outra; Sergio S Funari (Autor) Outra; Daniela Uhrikova (Autor) Outra

Revista

Título: Biophysical Journal

Vol. 101

Páginas: L20-L22

ISSN: 0006-3495

Editora: Elsevier

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 25 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-NT7

DOI: 10.1016/j.bpj.2011.06.038

Abstract (EN): The observation of a micellar cubic phase is reported for a mixture of an antimicrobial peptide from the Lactoferrin family, LFampin 265-284, and a model membrane system of dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (3:1), as derived from small-angle x-ray diffraction (SAXD) measurements. The system shows remarkable thermotropic polymorphism: the peptide disrupts the lipid bilayer, forming a cubic phase of the space group Pm 3n (t < 28 degrees C), and as the temperature increases it shows a complex phase behavior (not fully clarified by SAXD). The onset, volume fraction of each phase, and phase parameters are seen to vary with peptide/lipid ratio and temperature. The obtained SAXD data represent the first experimental evidence, to our knowledge, of a micellar cubic phase in the context of antimicrobial peptide/membrane interaction. We propose that the micellization of the membrane according to the carpet model, for long proposed as a possible mechanism of action, can go through the formation of a cubic micellar phase.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3