Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Raul G Saraiva (Autor) Outra; Joao Almeida Lopes (Autor) Outra; Jorge Machado (Autor) Outra; Paula Gameiro (Autor) FCUP; Maria J Feio (Autor) Outra

Revista

Título: Journal of Biophotonics

Vol. 7

Páginas: 392-400

ISSN: 1864-063X

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 11 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-009-GR3

DOI: 10.1002/jbio.201200131

Abstract (EN): Vibrational spectroscopy has long been used in bacterial identification with different levels of taxonomic discrimination but its true potential for intra-species differentiation remains poorly explored. Herein, both transmission Fourier-transform infrared (FTIR) and attenuated total reflectance (ATR)-FTIR spectroscopy are used to analyse E. coli strains that differ solely in their porin expression profile. In this previously unreported approach, the applicability of both FTIR-spectroscopy techniques is compared with the same collection of unique strains. ATR-FTIR spectroscopy proved to reliably distinguish between several E. coli porin mutants with an accuracy not replicated by FTIR in transmission mode (using previously optimized procedures). Further studies should allow the identification of the individual contribution of the single porin channel to the overall bacterial infrared spectrum and of molecular predictive patterns of porin alterations. [GRAPHICS] Model of the Gram-negative bacteria envelope [adapted from V. Teixeira, M. J. Feio and M. Bastos, Prog Lipid Res. 51(2), 149-177 (2012)].

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9