Nuclear localization of SYT, SSX and the synovial sarcoma-associated SYT-SSX fusion proteins

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

dosSantos, NR (Autor) Outra; deBruijn, DRH (Autor) Outra; Balemans, M (Autor) Outra; Janssen, B (Autor) Outra; Gartner, F (Autor) ICBAS; Lopes, JM (Autor) FMUP; deLeeuw, B (Autor) Outra; vanKessel, AG (Autor) Outra

Revista

Título: Human Molecular Genetics

Vol. 6

Páginas: 1549-1558

ISSN: 0964-6906

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 85 Citações

Scopus - 90 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-ARJ

DOI: 10.1093/hmg/6.9.1549

Abstract (EN): Synovial sarcoma is characterized by a prevalent chromosomal translocation, t(X;18)(p11;q11), As a result of this translocation the STT gene on chromosome 18 fuses to either the SSX1 or the SSX2 gene on the X chromosome, In this study, we generated polyclonal antibodies against the SYT and SSX2 proteins, These antibodies specifically detected both these proteins and the SYT-SSX fusion proteins in transfected COS-1 cell extracts, Indirect immunofluorescence analysis of COS-1 cells expressing tagged or untagged STT, SSX2, STT-SSX1 or SYT-SSX2 indicated that all these proteins are localized in the nucleus, excluding the nucleoli, The SSX2 protein exhibited a diffuse staining pattern whereas both the SYT and SYT-SSX proteins appeared in several nuclear dots, Similar nuclear dots were also detected in primary synovial sarcoma cells growing in a short-term in vitro culture, Double immunofluorescence in conjunction with confocal laser-scanning microscopy revealed that the STT and STT-SSX nuclear dots do not co-localize with known nuclear structures as e.g. coiled bodies, SC35 interchromatin granules or PML bodies, The similar nuclear localization patterns of STT and SYT-SSX suggest that the STT-SSX fusion proteins are directed to SYT-associated nuclear domains where an abnormal function may be exerted.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10