Using yeast to uncover the regulation of protein kinase C delta by ceramide

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Claudia Bessa (Autor) Outra; Clara Pereira (Autor) Outra; Mariana Leao (Autor) Outra; Claudia Maciel (Autor) Outra; Sara Gomes (Autor) Outra; Jorge Goncalves (Autor) FFUP; Manuela Corte Real (Autor) Outra; Vitor Costa (Autor) ICBAS; Lucilia Saraiva (Autor) FFUP

Revista

Título: FEMS Yeast Research

Vol. 13

Páginas: 700-705

ISSN: 1567-1356

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 3 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-006-JKZ

DOI: 10.1111/1567-1364.12069

Abstract (EN): The regulation of protein kinase C (PKC) isoforms by ceramide is still controversial. In this work, the yeast Saccharomyces cerevisiae was used as a model to elucidate the effect of ceramide on the activity of mammalian PKC isoforms. For that, isc1 cells, with a deletion in the pathway for ceramide production by hydrolysis of complex sphingolipids, individually expressing mammalian PKC, and were used. Contrary to PKC and , expression of PKC in isc1 cells exhibited a similar phenotype to that observed with wild-type yeast cells expressing PKC treated with a PKC activator, as phorbol 12-myristate 13-acetate (PMA), specifically a growth inhibition associated with a G2/M cell cycle arrest. Interestingly, in isc1 yeast cells expressing PKC this phenotype was completely abrogated in the presence of exogenous ceramide. Moreover, using a yeast-based assay previously developed for the screening of PKC inhibitors, it was also shown that, like the known PKC inhibitor NPC 15437, ceramide reduced the PMA-induced growth inhibition, supporting an inhibitory effect of ceramide on PKC. Altogether, these results may indicate that ceramide distinctly interfere with the activity of PKC, and . Most importantly, they showed that ceramide is an inhibitor of PKC.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6