Imidazole-grafted chitosan-mediated gene delivery: in vitro study on transfection, intracellular trafficking and degradation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Liliana R Pires (Autor) Outra; Hugo Oliveira (Autor) Outra; Cristina C Barrias (Autor) Outra; Paula Sampaio (Autor) Outra; Antonio J Pereira (Autor) Outra; Helder Maiato (Autor) FMUP; Sergio Simoes (Autor) Outra; Ana P Pego (Autor) FEUP

Revista

Título: Nanomedicine

Vol. 6 Nº 1

Páginas: 1499-1512

ISSN: 1743-5889

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 25 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Nanotecnologia

Outras Informações

ID Authenticus: P-002-JW3

DOI: 10.2217/nnm.11.51

Abstract (EN): Aim: To study the mechanism of transfection mediated by imidazole-grafted chitosan (CHimi) nanoparticles, to propose new strategies to control and improve the expression of a delivered gene in the context of regenerative medicine. Methods: Biochemical and microscopy methods were used to establish transfection efficiency and nanoparticle intracellular trafficking. The role of CHimi and degree of N-acetylation (DA) on transfection was explored. Results: CHimi was found to promote the expression of a delivered gene during a minimum 7-day period. Additionally, the production of a protein of interest could be upheld by consecutive transfections, without compromising cell viability. Transfection was found to be a time-dependent process, requiring CHimi-DNA complex disassembling. The DA was found to have an impact on transfection kinetics in line with the observation that the rate of lysozyme-mediated nanoparticle degradation increases with the polymer DA. Conclusion: The adjustment of the CH degradation rate can be used as a tool for tuning the expression of a gene delivered by CH-based nanoparticle systems.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14