Protein Matrices for Improved Wound Healing: Elastase Inhibition by a Synthetic Peptide Model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Andreia Vasconcelos (Autor) Outra; Ana Paula Pego (Autor) FEUP; Lara Henriques (Autor) Outra; Meriem Lamghari (Autor) FEUP; Artur Cavaco Paulo (Autor) Outra

Revista

Título: Biomacromolecules

Vol. 11

Páginas: 2213-2220

ISSN: 1525-7797

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 23 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-34N

DOI: 10.1021/bm100537b

Abstract (EN): The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase-antielastase imbalance found on chronic wounds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8