Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII(7-10))

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Amaral, IF (Autor) FEUP; Neiva, I (Autor) Outra; Ferreira da Silva, FF (Autor) Outra; Sousa, SR (Autor) Outra; Piloto, AM (Autor) Outra; Lopes, CDF (Autor) Outra; Barbosa, MA (Autor) ICBAS; Kirkpatrick, CJ (Autor) Outra; Pego, AP (Autor) FEUP

Revista

Título: Acta Biomaterialia

Vol. 9

Páginas: 5643-5652

ISSN: 1742-7061

Editora: Elsevier

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 15 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

Outras Informações

ID Authenticus: P-002-04E

DOI: 10.1016/j.actbio.2012.10.029

Abstract (EN): The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4% and 15%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII(7-10)). Immobilized rhFNIII(7-10) was characterized in terms of amount (I-125-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII(7-10) with rhFNIII(7-10) concentration, and, for the same concentration, higher amounts of rhFNIII(7-10) on DA 4% compared with DA 15%. Moreover, rhFNIII(7-10) concentrations as low as 5 and 20 mu g ml(-1) in the coupling reaction were shown to provide DA 4% and 15% scaffolds, respectively, with levels of exposed cell-binding domains exceeding those observed on the control (DA 4% scaffolds incubated in a 20 mu g ml(-1) human fibronectin solution). These grafting conditions proved to be effective in mediating EC adhesion/cytoskeletal organization on CH with DA 4% and 15%, without affecting the endothelial angiogenic potential. rhFNIII(7-10) grafting to CH could be a strategy of particular interest in tissue engineering applications requiring the use of endothelialized porous matrices with tunable degradation rates.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10