HPLC-DAD-ESI/MSn analysis of phenolic compounds for quality control of Grindelia robusta Nutt. and bioactivities

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Federico Ferreres (Autor) Outra; Clara Grosso (Autor) Outra; Angel Gil Izquierdo (Autor) Outra; Patricia Valentao (Autor) FFUP; Carolina Azevedo (Autor) Outra; Paula B Andrade (Autor) FFUP

Revista

Título: Journal of Pharmaceutical and Biomedical Analysis

Vol. 94

Páginas: 163-172

ISSN: 0731-7085

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 17 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-009-HXP

DOI: 10.1016/j.jpba.2014.01.046

Abstract (EN): The phenolic composition of herbal tea (HT) and hydromethanolic extract (HME) obtained from Grindelia robusta Nutt. was studied by HPLC-DAD-ESI/MSn. Thirty six flavonoids and hydroxycinnamic acids were detected, from which thirty are described for the first time in this species. Quantification by HPLC-DAD showed that diosmetin-7-0-glucuronide-3'-0-pentoside + apigenin-7-0-glucuronide-4'-0-pentoside, apigenin-7-0-glucuronide + diosmetin-7-0-glucuronide and 3,5-dicaffeoylquinic acid + 3,4-dicaffeoylquinic acid were the major compounds. Since the health-promoting effects of natural phenolic compounds against brain disorders is of increasing interest, HT and HME were also tested against oxygen and nitrogen reactive species and against enzymes related with Alzheimer's disease and depression. Extracts displayed strong in vitro scavenging activity and monoamine oxidase-A (MAO-A) inhibitory activity. The anti-MAO-A capacity was observed at non-toxic concentrations for SH-SY5Y human neuroblastoma cell line, reinforcing the benefits of G. robusta HT. However, no protection against hydrogen peroxide treatment was observed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10