Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Raquel Catarino (Autor) Outra; Maria M Ferreira (Autor) Outra; Helena Rodrigues (Autor) Outra; Ana Coelho (Autor) Outra; Ana Nogal (Autor) Outra; Abreu Sousa (Autor) Outra; Rui Medeiros (Autor) ICBAS

Revista

Título: DNA and Cell Biology

Vol. 27

Páginas: 415-421

ISSN: 1044-5498

Editora: Mary Ann Liebert

Indexação

ISI Web of Knowledge - 98 Citações

Scopus - 100 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-X6M

DOI: 10.1089/dna.2008.0744

Abstract (EN): Aim: To determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR quantification methodology. Methods: Our standard protocol for quantification of cell-free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. Results: We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs. 77.06 ng/mL, p < 0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery ( 105.2 vs. 59.0 ng/mL, p 0.001). Increased plasma cell-free DNA concentration was a strong risk factor for breast cancer, conferring an increased risk for the presence of this disease ( OR, 12.32; 95% CI, 2.09-52.28; p < 0.001). Conclusions: Quantification of circulating DNA by real-time PCR may be a good and simple tool for detection of breast cancer with a potential to clinical applicability together with other current methods used for monitoring the disease.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7