Enzymic flow injection determination of free L-carnitine in infant formulae

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

Ferreira, IMPLVO (Autor) FFUP; Macedo, MN (Autor) Outra; Ferreira, MA (Autor) Outra

Revista

Título: The Analyst

Vol. 122

Páginas: 1539-1541

ISSN: 0003-2654

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 14 Citações

Scopus - 17 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-A5H

DOI: 10.1039/a704903j

Abstract (EN): Infant formula samples mere analysed to determine their free L-carnitine content by using flow injection (FI) with an incorporated immobilised carnitine acetyltransferase bioreactor. The methodology was based on the spectrophotometric determination through its reaction with carnitine acetyltransferase coupled with acetyl coenzyme A (acetyl-CoA) and dithiobenzoate. The merging zones technique was used to minimise acetyl CoA consumption. Linearity was observed over the concentration range 10-80 mg l(-1) with L-carnitine as standard (r = 0.9998) and the rate of analysis was 50 h(-1) infant formula samples. The enzymic FI method afforded a low RSD (2.2%). Comparisons were made with other methods of known accuracy. The enzymic reactors were stable for 3 months when used daily at the optimum pH.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3