Optimization and validation of a method based in a QuEChERS procedure and gas chromatography-mass spectrometry for the determination of multi-mycotoxins in popcorn

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Ferreira, I (Autor) FFUP; Fernandes, JO (Autor) FFUP; Cunha, SC (Autor) Outra

Revista

Título: Food Control

Vol. 27

Páginas: 188-193

ISSN: 0956-7135

Editora: Elsevier

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 43 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-002-6S3

DOI: 10.1016/j.foodcont.2012.03.014

Abstract (EN): In this work a gas chromatography-mass spectrometry (GC-MS) method for multi-mycotoxins (deoxynivalenol, nivalenol, 15-acetyl-deoxynivalenol, fusarenon X and zearalenone) determination in unpopped and popped popcorn was optimized and validated. Fast sample preparation procedure is based on a pre-extraction of fat compounds with n-hexane, extraction of target analytes with acetonitrile and removing of matrix co-extracts by a simple dispersive solid phase extraction employing MgSO4 and C18 sorbents followed by sylilation of the analytes and further GC-MS analysis. The recoveries in spiked samples (20 and 100 mu g/kg) ranged from 61 to 118% in unpopped popcorn and from 65 to 89% in popped popcorn, with relative standard deviations lower than 18%. Limits of detection and quantification were lower than 65 mu g/kg and 196 mu g/kg, respectively. The method was applied in 30 popcorn samples commercialized in Portugal; one sample was positive for nivalenol and zearalenone, at concentrations below the maximum permissible level.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6