A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Rosa Pereira (Autor) Outra; Tommy Julianto (Autor) Outra; Peh Kim Ang (Autor) Outra; Sharon Sheue Nee Ling (Autor) Outra; Carlos Mauricio Barbosa (Autor) FFUP; Kah Hay Yuen (Autor) Outra; Abu Bakar Abdul Majeed (Autor) Outra

Revista

Título: Chromatographia

Vol. 71 Nº 5/6

Páginas: 373-381

ISSN: 0009-5893

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 3 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-8G6

DOI: 10.1365/s10337-010-1481-5

Abstract (EN): A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min(-1) and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R (2) a parts per thousand yen 0.999), precise and accurate in a concentration range of 0.2-50.0 mu g mL(-1). The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles.

Idioma: Inglês

Tipo (Avaliação Docente): Científica