Carbon dots obtained using hydrothermal treatment of formaldehyde. Cell imaging in vitro

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Algarra, M (Autor) Outra; Perez Martin, M (Autor) Outra; Cifuentes Rueda, M (Autor) Outra; Jimenez Jimenez, J (Autor) Outra; Esteves da Silva, JCGE (Autor) FCUP; Bandosz, TJ (Autor) Outra; Rodriguez Castellon, E (Autor) Outra; Lopez Navarrete, JTL (Autor) Outra; Casado, J (Autor) Outra

Revista

Título: Nanoscale

Vol. 6

Páginas: 9071-9077

ISSN: 2040-3364

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 78 Citações

Scopus - 86 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia dos materiais

Outras Informações

ID Authenticus: P-009-PAA

DOI: 10.1039/c4nr01585a

Abstract (EN): Highly phototuminescent carbon dots have been prepared in a one step procedure by hydrothermal treatment of formaldehyde at 180 degrees C. They show green fluorescence under UV light exposure and emission spectra are centered at 440 nm. Fluorescence lifetimes comprise between 0.7 and 2.70 ns, when the synthesis process lasted for 1-7 days. TEM images of nanoparticles showed a homogeneous size/shape distribution. When the thermal treatment process was carried out for a long time (30 days) formation of aggregates occurred. Carbon dots were further analyzed using H-1 and C-13-NMR, Raman and FTIR spectroscopy techniques and XPS. Cell imaging of nanopartictes was carried out by using mouse MC3T3-E1 pre-osteoblasts as a model. The nanoparticles were selectively localized in the cytoplasm without further functionalization and could be realized by cellular phagocytosis, so that the fluorescence of these can be used for live cell imaging in vitro.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7