Coffee silverskin: A possible valuable cosmetic ingredient

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Francisca Rodrigues (Autor) Outra; Ana Palmeira de Oliveira (Autor) Outra; Jose das Neves (Autor) Outra; Bruno Sarmento (Autor) Outra; Helena Amaral, MH (Autor) FFUP; Beatriz B P P Oliveira (Autor) FFUP

Revista

Título: Pharmaceutical Biology

Vol. 53

Páginas: 386-394

ISSN: 1388-0209

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 61 Citações

Scopus - 68 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia médica

Outras Informações

ID Authenticus: P-00A-5TM

DOI: 10.3109/13880209.2014.922589

Abstract (EN): Context: Currently, there is a great tendency in cosmetic area to use natural extracts. Coffee silverskin (CS) is the most abundant solid by-product generated during roasting of coffee processing. Objectives: To evaluate different CS extracts as promising cosmetic ingredients, regarding antioxidant, antimicrobial, and cytotoxic properties. Materials and methods: Aqueous, hydroalcoholic and ethanolic CS extracts were obtained by an environmentally friendly procedure considering costs and pollution. Extracts were characterized for total phenolic and flavonoid contents (TPC and TFC, respectively), antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), antimicrobial activity expressed as minimal inhibitory concentration (MIC) and cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and lactate dehydrogenase (LDH) assays in two skin cell lines (fibroblasts and keratinocytes). Results: The TPC of extracts was 18.33-35.25 mg of gallic acid equivalents per g of material on a dry basis (mg GAE/g db). The TFC of extracts was 1.08-2.47 mu g cathechin equivalents per g dry material (mu g CE/g db). The antioxidant activity was high, with values ranging between 95.95 and 216.40 mu mol Fe2+/g for aqueous and alcoholic samples, respectively. Preliminary assays for antimicrobial potential showed that extracts display antibacterial activity. The MIC varied from 31.3 to 250 mu g/mL for Gram-positive, and from 31.3 to 1000 mu g/mL for Gram-negative. Extracts did not affect in vitro cell viability, with values near 100% in all concentrations tested. Conclusion: Results seem show that CS is a safe source of natural antioxidants with antifungal and antibacterial activity and no cytotoxicity, with potential usefulness for cosmetic applications.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9