Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Sarmento, B (Autor) Outra; Ferreira, DC (Autor) FFUP; Jorgensen, L (Autor) Outra; van de Weert, M (Autor) Outra

Revista

Título: European Journal of Pharmaceutics and Biopharmaceutics

Vol. 65

Páginas: 10-17

ISSN: 0939-6411

Editora: Elsevier

Indexação

ISI Web of Knowledge - 135 Citações

Scopus - 170 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-004-D3R

DOI: 10.1016/j.ejpb.2006.09.005

Abstract (EN): The aim of the present study was to probe the structural integrity of insulin after being entrapped into chitosan/alginate nanoparticles produced by ionotropic polyelectrolyte pre-gelation. By manipulating the alginate:chitosan mass ratio and the pH during nanoparticle production, desired nanoparticles with a mean size of 850 (+/- 88) nm and insulin association efficiency of 81 (+/- 2)% were obtained. Insulin secondary structure was assessed by Fourier transform infrared (FTIR) and circular dichroism (CD) after entrapment into nanoparticles and after release from the particles under gastrointestinal simulated conditions. FTIR second-derivative spectra and area-overlap compared to an insulin standard confirmed that no significant conformational changes of insulin occurred in terms of alpha-helix and beta-sheet content. Far-UV-CD spectra corroborated the preservation of insulin structure during the nanoparticle production procedure. The presented nanoparticulate system is a promising carrier for insulin oral delivery since it preserves insulin structure and therefore also, potentially, its bioactivity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8