Improving Oral Absorption of Samon Calcitonin by Trimyristin Lipid Nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Martins, S (Autor) Outra; Silva, AC (Autor) Outra; Ferreira, DC (Autor) FFUP; Souto, EB (Autor) Outra

Revista

Título: Journal of Biomedical Nanotechnology

Vol. 5

Páginas: 76-83

ISSN: 1550-7033

Editora: American Scientific Publishers

Indexação

ISI Web of Knowledge - 47 Citações

Scopus - 53 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Nanotecnologia

Outras Informações

ID Authenticus: P-003-NGP

DOI: 10.1166/jbn.2009.443

Abstract (EN): Solid lipid nanoparticles (SLN) composed of trimyristin (solid lipid) and poloxamer 407 (surfactant) were prepared by a w/o/w emulsion technique for the incorporation of Salmon calcitonin, and further explored as protein carriers for oral delivery. Trimyristin SLN showed a mean size diameter of 200 nm with an association efficiency for calcitonin of approx. 86%. The morphology of SLN was investigated by cryo-SEM and by AFM, revealing spheroid shape SLN with a smooth surface. The in vitro release of calcitonin occurred for a period of 8 h, under both gastric and intestinal simulated pH conditions, predicting suitable properties for oral administration. The pharmacological activity of the protein was evaluated following oral dosage of calcitonin-loaded SLN in rats. SLN lowered the basal blood calcium levels by up to 20% with 500 IU/kg dose sustaining hypocalcaemia over 8 h. The results indicate that incorporation of Salmon calcitonin into trimyristin SLN is a key factor for the improvement of the efficiency of such carriers for oral delivery of proteins.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8