Direct determination of copper in urine using a sol-gel optical sensor coupled to a multicommutated flow system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Jeronimo, PCA (Autor) Outra; Araujo, AN (Autor) FFUP; Montenegro, MCBSM (Autor) FFUP; Pasquini, C (Autor) Outra; Raimundo, IM (Autor) Outra

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 380 Nº 1

Páginas: 108-114

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 42 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-8KB

DOI: 10.1007/s00216-004-2718-7

Abstract (EN): In this work, a multicommutated flow system incorporating a sol-gel optical sensor is proposed for direct spectrophotometric determination of Cu(II) in urine. The optical sensor was developed by physical entrapment of 4-(2-pyridylazo)resorcinol (PAR) in sol-gel thin films by means of a base-catalysed process. The immobilised PAR formed a red 2:1 complex with Cu(II) with maximum absorbance at 500 nm. Optical transduction was based on a dual-colour light-emitting diode (LED) (green/red) light source and a photodiode detector. The sensor had optimum response and good selectivity towards Cu(II) at pH 7.0 and its regeneration was accomplished with picolinic acid. Linear response was obtained for Cu(II) concentrations between 5.0 and 80.0 mug L-1, with a detection limit of 3.0 mug L-1 and sampling frequency of 14 samples h(-1). Interference from foreign ions was studied at a 10:1 (w/w) ion:Cu(II) ratio. Results obtained from analysis of urine samples were in very good agreement with those obtained by inductively coupled plasma mass spectrometry (ICP-MS); there was no significant differences at a confidence level of 95%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7