Lipidomic Approach Towards Deciphering Anandamide Effects in Rat Decidual Cell

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Marta Almada (Autor) Outra; Rosario Domingues, MR (Autor) Outra; Luisa Doria, ML (Autor) Outra; Bruno M Fonseca (Autor) Outra; Natercia A Teixeira (Autor) FFUP; Georgina Correia da Silva (Autor) FFUP

Revista

Título: Journal of Cellular Physiology

Vol. 230

Páginas: 1549-1557

ISSN: 0021-9541

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 5 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-00A-9G6

DOI: 10.1002/jcp.24901

Abstract (EN): Altered phospholipid (PL) metabolism has been associated with pregnancy disorders. Moreover, lipid molecules such as endocannabinoids (eCBs) and prostaglandins (PGs) are important mediators of reproductive events. In humans, abnormal decidualization has been linked with unexplained infertility, miscarriage and endometrial pathologies. Anandamide (AEA), the major eCB, induces apoptosis in rat decidual cells. In this study, the PL profile of rat decidual cells was characterized by a Mass spectrometry (MS) based lipidomic approach. Furthermore, we analyzed a possible correlation between changes in PL of rat decidual cells' membrane and AEA-induced apoptosis. We found an increase in phosphatidylserine and a reduction of cardiolipin and phophatidylinositol relative contents. In addition, we observed an increase in the content of alkyl(alkenyl) acylPL, plasmalogens, and of long chain fatty acids especially with high degrees of unsaturation, as well as an increase in lipid hydroperoxides in treated cells. These findings provide novel insights on deregulation of lipid metabolism by anandamide, which may display further implications in decidualization process. J. Cell. Physiol. 230: 1549-1557, 2015. (c) 2014 Wiley Periodicals, Inc., A Wiley Company

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9