Immunoreactive pattern of Staphylococcus epidermidis biofilm against human whole saliva

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Virgínia Carvalhais (Autor) Outra; Francisco Amado (Autor) Outra; Frederico Cerveira (Autor) Outra; Rita Ferreira (Autor) Outra; Manuel Vilanova (Autor) ICBAS; Nuno Cerca (Autor) Outra; Rui Vitorino (Autor) Outra

Revista

Título: Electrophoresis

Vol. 36 Nº 9-10

Páginas: 1228-1233

ISSN: 0173-0835

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 3 Citações

ISI Web of Science

Scopus - 3 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-00G-4VP

DOI: 10.1002/elps.201500043

Abstract (EN): Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6