Detection of prognostic significant translocations in childhood acute lymphoblastic leukaemia by one-step multiplex reverse transcription polymerase chain reaction

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

cerveira, n (Autor) Outra; ferreira, s (Autor) Outra; doria, s (Autor) FMUP; veiga, i (Autor) Outra; ferreira, f (Autor) Outra; martz, jm (Autor) Outra; marques, m (Autor) Outra; castedo, s (Autor) FMUP

Revista

Título: British Journal of Haematology

Vol. 109

Páginas: 638-640

ISSN: 0007-1048

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 11 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-000-ZSH

DOI: 10.1046/j.1365-2141.2000.02051.x

Abstract (EN): The search for chromosomal translocations in df novo cases of childhood acute lymphoblastic leukaemia (ALL) is crucial for the selection of the appropriate therapeutic protocol. In this work, we describe a new method - one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) - to screen for prognostic significant translocations in childhood ALL. Our approach involves a single PCR reaction for the simultaneous detection of the molecular rearrangements resulting from the t(9:22), t(12;21), t(4;11) and t(1;19), with a turnaround time of less than 24 h. This assay proved to be highly sensitive, specific, reproducible and easy to implement in a routine genetics laboratory.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3