An alternative respiratory pathway on Candida krusei: implications on susceptibility profile and oxidative stress

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

costa-de-oliveira, s (Autor) FMUP; sampaio-marques, b (Autor) Outra; barbosa, m (Autor) Outra; ricardo, e (Autor) Outra; pina-vaz, c (Autor) FMUP; ludovico, p (Autor) Outra; rodrigues, ag (Autor) FMUP

Revista

Título: FEMS Yeast Research

Vol. 12

Páginas: 423-429

ISSN: 1567-1356

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 21 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-96G

DOI: 10.1111/j.1567-1364.2012.00789.x

Abstract (EN): Our aim was to detect the presence of an alternative oxidase (AOX) in Candida krusei clinical strains and its influence on fluconazole susceptibility and in reactive oxygen species (ROS) production. Candida krusei clinical isolates were tested to evaluate the presence of AOX. Debaromyces hansenii 2968 (AOX positive) and Saccharomyces cerevisiae BY4742 (AOX negative) were used as control strains. Measurements of oxygen consumption were performed in the presence of 1 mM KCN, an inhibitor of the classical respiratory chain, and 5 mM salicylhydroxamic acid (SHAM). AOX expression was monitored by Western blotting using an AOX monoclonal antibody. Interactions between fluconazole and SHAM were performed using checkerboard assay. ROS production was evaluated in the presence of SHAM plus fluconazole, H2O2, menadione, or plumbagin. AOX was present in all C. krusei tested. The combination of fluconazole with SHAM resulted in an indifferent effect. In the presence of SHAM, the treatment with ROS inductors or fluconazole increased ROS production, except in the AOX-negative strain. An alternative respiratory pathway resistant to cyanide is described for the first time as a characteristic of C. krusei species. This AOX is unrelated to fluconazole resistance; however, it protects C. krusei from oxidative stress.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7