Comparative ultrastructural analysis of diestrous and anestrous canine Grade 1 cumulus-oocyte complexes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

lopes, g (Autor) ICBAS; rocha, a (Autor) ICBAS; sousa, m (Autor) ICBAS

Revista

Título: Animal Reproduction Science

Vol. 122

Páginas: 244-252

ISSN: 0378-4320

Editora: Elsevier

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências agrárias > Ciências veterinárias

Outras Informações

ID Authenticus: P-003-0AA

DOI: 10.1016/j.anireprosci.2010.08.018

Abstract (EN): The objective of the present study was to characterize, by means of transmission electron microscopy, immature Grade 1 cumulus-oocyte complexes (COCs) obtained from ovaries collected from bitches at diestrus and anestrus, after routine ovariohysterectomy. Cumulus-oocyte complexes were recovered after slicing the ovarian cortex and Grade 1 COCs were selected and prepared for transmission electron microscopy. All oocytes were at germinal vesicle stage in two different configurations. In 29 out of 37 COCs, oocytes presented a central or eccentric located nucleus (GVc), frequently containing a reticulated nucleolus, with a predominance of profound follicular cell processes (FCP), abundant endoplasmic reticulum (ER) and a moderated number of lipid droplets. In eight out of 37 COCs, oocytes had a peripheral located nucleus (Gyp) containing small compact nucleolus, a thin perivitelline space, both superficial and profound FCP, moderate ER content and abundant lipid droplets. A higher (p < 0.05) proportion of Gyp was found in oocytes collected in diestrus (36.8% - 7 out of 19) than in anestrus (5.6% - 1 out of 18). In addition, distinct ultrastructure characteristics among GVc associated with estrous phase were noted. Furthermore, a number of structures, some of which had not been reported before, were present in canine prophase I oocytes at variable frequencies. The evident variation in the presence, quantity and distribution of cell organelles in canine immature Grade 1 oocytes is discussed in relation to the biological phase of the oocyte and the reproductive stage of the donor bitch.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9