Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: Bioactivity and osteoblast-like cell behaviour

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

M. S. Laranjeira (Autor) Outra; M. H. Fernandes (Autor) FMDUP; F. J. Monteiro (Autor) FEUP

Revista

Título: Journal of Biomedical Materials Research - Part A

Vol. 95A Nº 3

Páginas: 891-900

ISSN: 1549-3296

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 23 Citações

ISI Web of Science

Scopus - 32 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-003-0KX

DOI: 10.1002/jbm.a.32916

Abstract (EN): To modulate the biological response of implantable granules, two types of bioactive porous granules composed of nanostructured-hydroxyapatite (HA) agglomerates and microstructured-HA, respectively, were prepared using a polyurethane sponge impregnation and burnout method. The resulting granules presented a highly porous structure with interconnected porosity. Both types of granules were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEMI, and mercury intrusion porosimetry. Results showed that nanostructed-HA granules presented higher surface area and porosity than microstructured-HA granules. In vitro testing using MG63 human osteoblast-like cells showed that on both types of surfaces cells were able to adhere, proliferate, and migrate through the macropores, and a higher growth rate was achieved on nanostructured-HA granules than on microstructured-HA granules (76 and 40%, respectively). In addition, these cells maintained similar expression levels of osteoblastic-associated markers namely collagen type I, alkaline phosphatase, bone morphogenetic protein-2, macrophage colony-stimulating factor, and osteoprotegerin. These innovative nanostructured-HA granules may be considered as promising bioceramic alternative matrixes for bone regeneration and drug release application. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 891-900,2010.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10