Injectable Gellan Gum Hydrogels with Autologous Cells for the Treatment of Rabbit Articular Cartilage Defects

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Oliveira, JT (Autor) Outra; Gardel, LS (Autor) Outra; Rada, T (Autor) Outra; Martins, L (Autor) Outra; Gomes, ME (Autor) Outra; Rui Reis (Autor) Outra

Revista

Título: Journal of Orthopaedic Research

Vol. 28

Páginas: 1193-1199

ISSN: 0736-0266

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 105 Citações

Scopus - 115 Citações

Outras Informações

ID Authenticus: P-003-3MQ

DOI: 10.1002/jor.21114

Abstract (EN): In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellan gum with encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASC + GF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Full-thickness articular cartilage defects were created and the gellan gum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASC + GF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASC + GF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cells may constitute a valid alternative to currently used articular chondrocytes. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1193-1199, 2010

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7