Spectrophotometric Determination of Thiocyanate in Human Saliva Employing Micropumping Multicommutation Flow System

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Joilton J Silva Junior (Autor) Outra; Marcelo A Farias (Autor) Outra; Valdinete L Silva (Autor) Outra; Maria C B S M Montenegro (Autor) FFUP; Alberto N Araujo (Autor) FFUP; Andre F Lavorante (Autor) Outra; Ana Paula S Paim (Autor) Outra

Revista

Título: Spectroscopy Letters

Vol. 43

Páginas: 213-219

ISSN: 0038-7010

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 6 Citações

ISI Web of Science

Scopus - 11 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Física

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-003-CYV

DOI: 10.1080/00387010903287094

Resumo (PT): A procedure based on a multicommutated approach for the determination of thiocyanate (SCN-) in human saliva is described. The method was based on the reaction of Fe(III) with SCN-, producing a red complex that was monitored at 480 nm. Under optimum experimental conditions, a linear response ranging from 0.5 to 10.0 m mol L-1 SCN- (R = 0.9991), a relative standard deviation of 1.0% (n = 10) for a sample 5 m mol L-1 SCN-, a detection limit (3σ criterion) of 50 µ mol L-1, a sampling rate of 60 determinations per hour, reagent consumption of 0.02 mg Fe(III) per determination, and a waste generation of 1.6 mL per determination were achieved. <br> <br> Keywords: human saliva; multicommutation flow analysis; multipumping; spectrophotometry; thiocyanate <br> <a target="_blank" href="http://prod.informaworld.com/smpp/content~db=all~content=a920928143~frm=titlelink">Texto integral </a> <br> <br>

Abstract (EN): A procedure based on a multicommutated approach for the determination of thiocyanate (SCN-) in human saliva is described. The method was based on the reaction of Fe(III) with SCN-, producing a red complex that was monitored at 480nm. Under optimum experimental conditions, a linear response ranging from 0.5 to 10.0mmolL-1 SCN- (R=0.9991), a relative standard deviation of 1.0% (n=10) for a sample 5mmolL-1 SCN-, a detection limit (3 sigma criterion) of 50 mu molL-1, a sampling rate of 60 determinations per hour, reagent consumption of 0.02mg Fe(III) per determination, and a waste generation of 1.6mL per determination were achieved.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7