Unveiling the bioactive potential of Actinomycetota from the Tagus River estuary

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

dos Santos, JDN (Autor) Outra; Eugenia Pinto (Autor) FFUP; Martín, J (Autor) Outra; Vicente, F (Autor) Outra; Reyes, F (Autor) Outra; lage, om (Autor) FCUP

Revista

Título: International Microbiology

ISSN: 1139-6709

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-V91

DOI: 10.1007/s10123-024-00483-0

Abstract (EN): The increase in global travel and the incorrect and excessive use of antibiotics has led to an unprecedented rise in antibiotic resistance in bacterial and fungal populations. To overcome these problems, novel bioactive natural products must be discovered, which may be found in underexplored environments, such as estuarine habitats. In the present work, estuarine actinomycetotal strains were isolated with conventional and iChip techniques from the Tagus estuary in Alcochete, Portugal, and analysed for different antimicrobial bioactivities. Extracts were produced from the isolated cultures and tested for bioactivity against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Aspergillus fumigatus ATCC 240305, Candida albicans ATCC 10231 and Trichophyton rubrum FF5. Furthermore, bioactive extracts were subjected to dereplication by high-performance liquid chromatography (HPLC) and high-resolution mass spectrometry (HRMS) to putatively identify their chemical components. In total, 105 isolates belonging to 3 genera were obtained. One which was isolated, MTZ3.1( T), represents a described novel taxon for which the name Streptomyces meridianus was proposed. Regarding the bioactivity testing, extracts from 12 strains proved to be active against S. aureus, 2 against E. coli, 4 against A. fumigatus, 3 against C. albicans and 10 against T. rubrum. Dereplication of bioactive extracts showed the presence of 28 known bioactive molecules, 35 hits have one or more possible matches in the DNP and 18 undescribed ones. These results showed that the isolated bacteria might be the source of new bioactive natural products.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16