Evidence for an urethro-vesical crosstalk mediated by serotonin

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Coelho, A (Autor) Outra; Oliveira, R (Autor) Outra; Cavaleiro, H (Autor) Outra; Cruz C.D. (Autor) FMUP; Francisco Cruz (Autor) FMUP

Revista

Título: Neurourology and Urodynamics

Vol. 37

Páginas: 2389-2397

ISSN: 0733-2467

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00P-T0Y

DOI: 10.1002/nau.23590

Abstract (EN): AimMethodsTo study the contribution of urethral serotonin for the urethro-vesical crosstalk Urethane-anesthetized female rats and TPH1(-/-) mice underwent isovolumetric or urethral-opened cystometries during intravesical or intraurethral infusion of saline or serotonin solutions. Human and rat bladders and urethrae were immunoreacted against serotonin and neuronal markers. Serotonin concentration and TPH1 mRNA were determined in rat tissue by HPLC and qPCR. ResultsConclusionsIn rats, under isovolumetric conditions, intraurethral serotonin infusion, but not saline, evoked bladder contractions. This was abolished by urethral anesthesia and by treatment with serotonin receptor antagonists. Serotonin infusion into the bladder had no effect. Under urethral-opened conditions, serotonin infusion reduced the frequency and increased the amplitude of reflex voiding contractions, compared to saline infusion. TPH1(-/-) mice, under urethral-opened conditions, exhibited increased frequency and reduced amplitude of voiding contractions compared to WT. Serotonin concentration and TPH1 mRNA expression were higher in the urethra than in the bladder. Cells 5-HT+ were found in the human and rat urethral epithelium, close to a sub-epithelial network of cholinergic and sensory fibers, but not in the bladder. Serotonin, produced and released by urethral cells activates an urethro-vesical pathway that enhances bladder reflex contractions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9