LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

marques, sm (Autor) Outra; petushkov, vn (Autor) Outra; rodionova, ns (Autor) Outra; da silva, jcge (Autor) FCUP

Revista

Título: Journal of Photochemistry and Photobiology B: Biology

Vol. 102 Nº 3

Páginas: 218-223

ISSN: 1011-1344

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 13 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-TAJ

DOI: 10.1016/j.jphotobiol.2010.12.006

Abstract (EN): This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MSn fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6