Molecular interactions between Vitamin B12 and membrane models: A biophysical study for new insights into the bioavailability of Vitamin

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020-10

Autores

Maria Ramalho (Autor) FEUP; Stephanie Andrade (Autor) FEUP; Mauel A. N. Coelho (Autor) FEUP; Joana A. Loureiro (Autor) FEUP; Maria do Carmo Pereira (Autor) FEUP

Revista

Título: Colloids and Surfaces B: Biointerfaces

Vol. 194

Páginas: 1-8

ISSN: 0927-7765

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 6 Citações

ScienceDirect (Elsevier)

Outras Informações

ID Authenticus: P-00S-AH1

DOI: 10.1016/j.colsurfb.2020.111187

Abstract (EN): Vitamin B12 (VB12) deficiency is one of the most common malnutrition problems worldwide and is related to its poor bioavailability. The lipid composition of cell membranes and molecule-cell membrane lipid interactions are major factors affecting the bioavailability of nutrients. So, the study of these interactions may allow predicting the behavior of VB12 at cellular membranes and the effects on its activity. Thus, lipid vesicles with lipid composition similar to the majority of eukaryotic cell membranes were used as biomembrane models, and their interactions with VB12 molecules were evaluated. For that, different parameters were assessed such as the lipophilicity of VB12, its preferential location in the membrane and its effect on the physical properties of the bilayer. VB12 showed high affinity for the biological membranes, not inducing any biophysical changes in their properties. The interactions of VB12 with the membrane was affected by the complexity of the bilayer, since its increase in order and rigidity hinders the diffusion of molecules. Thus, the low bioavailability of VB12 is not related with its interactions with the biological membranes. © 2020 Elsevier B.V.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8