Acquired AmpC beta-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Ribeiro, TG (Autor) FFUP; Novais, A (Autor) Outra; Machado, E (Autor) Outra; Luisa Peixe (Autor) FFUP

Revista

Título: Pathogens

Vol. 9

Página Final: 273

Editora: MDPI

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Outras Informações

ID Authenticus: P-00S-1S6

DOI: 10.3390/pathogens9040273

Abstract (EN): We aimed to investigate the occurrence of acquired AmpC beta-lactamases (qAmpC), and characterize qAmpC-producing Enterobacteriaceae from different non-clinical environments in Portugal. We analysed 880 Enterobacteriaceae resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among Enterobacteriaceae from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing Escherichia coli from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013-2014 versus 0% in 2001-2004], which is in accordance with the trend observed in other European countries. CMY-2-producing E. coli belonged to B2(2)-ST4953 (n = 2, HH), A(0)-ST665 (n = 1, HH) or A(1)-ST48 (n = 1, UCC) clones. bla(CMY-2) was identified in non-typeable and IncA/C-2 plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing Enterobacteriaceae occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of bla(qAmpC) in non-clinical settings.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5