Transcription elongation rate has a tissue-specific impact on alternative cleavage and polyadenylation in Drosophila melanogaster

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Liu, XC (Autor) Outra; Freitas, J (Autor) Outra; Zheng, DH (Autor) Outra; Beatriz B P P Oliveira (Autor) FFUP; Hoque, M (Autor) Outra; Martins, T (Autor) Outra; Henriques, T (Autor) Outra; Tian, B (Autor) Outra; Moreira, A (Autor) Outra

Revista

Título: RNA

Vol. 23

Páginas: 1807-1816

ISSN: 1355-8382

Editora: Cold Spring Harbor Laboratory Press

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 26 Citações

Outras Informações

ID Authenticus: P-00N-7WM

DOI: 10.1261/rna.062661.117

Abstract (EN): Alternative polyadenylation (APA) is a mechanism that generates multiple mRNA isoforms with different 3'UTRs and/or coding sequences from a single gene. Here, using 3'region extraction and deep sequencing (3'READS), we have systematically mapped cleavage and polyadenylation sites (PASs) in Drosophila melanogaster, expanding the total repertoire of PASs previously identified for the species, especially those located in A-rich genomic sequences. Cis-element analysis revealed distinct sequence motifs around fly PASs when compared to mammalian ones, including the greater enrichment of upstream UAUA elements and the less prominent presence of downstream UGUG elements. We found that over 75% of mRNA genes in Drosophila melanogaster undergo APA. The head tissue tends to use distal PASs when compared to the body, leading to preferential expression of APA isoforms with long 3'UTRs as well as with distal terminal exons. The distance between the APA sites and intron location of PAS are important parameters for APA difference between body and head, suggesting distinct PAS selection contexts. APA analysis of the RpII215(C4) mutant strain, which harbors a mutant RNA polymerase II (RNAPII) with a slower elongation rate, revealed that a 50% decrease in transcriptional elongation rate leads to a mild trend of more usage of proximal, weaker PASs, both in 3'UTRs and in introns, consistent with the "first come, first served" model of APA regulation. However, this trend was not observed in the head, suggesting a different regulatory context in neuronal cells. Together, our data expand the PAS collection for Drosophila melanogaster and reveal a tissue-specific effect of APA regulation by RNAPII elongation rate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10