Detection of Aspergillus species in BACTEC blood cultures

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Rosa, C (Autor) Outra; Ricardo Araujo (Autor) FCUP; rodrigues, ag (Autor) FMUP; Pinto de Sousa, MI (Autor) Outra; pina-vaz, c (Autor) FMUP

Revista

Título: Journal of Medical Microbiology

Vol. 60 Nº (Pt12)

Páginas: 1467-1471

ISSN: 0022-2615

Editora: Microbiology Society

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 16 Citações

Outras Informações

ID Authenticus: P-002-M40

DOI: 10.1099/jmm.0.030601-0

Abstract (EN): Invasive aspergillosis is associated with high morbidity and mortality rates; nevertheless, blood cultures almost invariably yield a negative result. The recovery and detection time of Aspergillus fumigatus, Aspergillus flavus and Aspergillus terreus were studied in BACTEC Plus Aerobic/F, Mycosis-IC/F and Myco/F Lytic vials, incubated in the BACTEC 9240 and 9000 MB automated systems. Two different approaches were used for subculture in solid medium: (i) the routine method, using a sterile airway needle/subculture unit, and (ii) a novel procedure, using instead a tuberculin disposable syringe and collecting a larger aliquot (100 mu l), following vigorous agitation of the vials. A. fumigatus was detected at inoculum concentrations of >3 conidia per 10 ml after 21-40 h, in both BACTEC Plus Aerobic/F and BACTEC Mycosis-IC/F vials. A few more hours were needed to detect A. flavus and A. terreus. The novel subculture procedure of BACTEC culture vials on solid medium resulted in several positive results that were not detected by the routine sampling procedure. BACTEC Plus Aerobic/F vials show an advantage particularly in patients under antifungal treatment. In cases of polymicrobial bloodstream infections (concurrent bacterial growth), the inoculation of blood samples into a BACTEC Mycosis-IC/F vial achieved the best results. Further multicentre studies are needed to validate this improved automated detection of Aspergillus spp. from blood cultures in clinical laboratories, as this diagnostic procedure allows antifungal susceptibility testing of moulds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5