Interactions between calcium, phosphate, and albumin on the surface of titanium

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Lima, J (Autor) Outra; Sousa, SR (Autor) Outra; Ferreira, A (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP

Revista

Título: Journal of Biomedical Materials Research

Vol. 55

Páginas: 45-53

ISSN: 0021-9304

Editora: Heterocorporation

Indexação

ISI Web of Knowledge - 50 Citações

Scopus - 48 Citações

Outras Informações

ID Authenticus: P-000-VZ4

DOI: 10.1002/1097-4636(200104)55:1<45::aid-jbm70>3.0.co;2-0

Abstract (EN): The deposition of calcium phosphate on chemically polished commercially pure titanium immersed in Hank's balanced salt solution (HBSS) with bovine serum albumin (BSA) (concentrations 0 and 4 mg/mL) has been I-125 labeling of investigated. Electrochemical techniques, albumin, scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy were used. A tricalcium phosphate layer with a thickness of ca. 1 mum was formed for periods of immersion in HBSS ranging between 1 and 2 weeks. A concentration of 4 mg/mL of BSA prevented its formation, even for periods as long as 1 month. In the absence of BSA, the electrochemical behavior of titanium specimens was significantly affected by the length of immersion time, reflecting the changes that slowly occur on their surface. In the presence of BSA, the surfaces maintained most of their original electrochemical activity. Surface studies have shown that calcium and phosphate become incorporated in the surface at very early stages of immersion. Albumin, which was rapidly adsorbed on titanium, was slowly desorbed when titanium was placed in HBSS. Protein and phosphate may coexist on the same surface, but initially adsorbed albumin molecules prevent the precipitation of a thick layer of tricalcium phosphate. (C) 2000 John Wiley & Sons, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9