CHITOSAN DRIVES ANTI-INFLAMMATORY MACROPHAGE POLARISATION AND PRO-INFLAMMATORY DENDRITIC CELL STIMULATION

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Oliveira, MI (Autor) Outra; Santos, SG (Autor) Outra; Oliveira, MJ (Autor) FCUP; Torres, AL (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP

Revista

Título: European Cells and Materials

Vol. 24

Páginas: 136-153

ISSN: 1473-2262

Editora: AO Research Institute Davos

Indexação

ISI Web of Knowledge - 69 Citações

Scopus - 26 Citações

Outras Informações

ID Authenticus: P-002-88S

Abstract (EN): Macrophages and dendritic cells (DC) share the same precursor and play key roles in immunity. Modulation of their behaviour to achieve an optimal host response towards an implanted device is still a challenge. Here we compare the differentiation process and polarisation of these related cell populations and show that they exhibit different responses to chitosan (Ch), with human monocyte-derived macrophages polarising towards an anti-inflammatory phenotype while their DC counterparts display pro-inflammatory features. Macrophages and DC, whose interactions with biomaterials are frequently analysed using fully differentiated cells, were cultured directly on Ch films, rather than exposed to the polymer after complete differentiation. Ch was the sole stimulating factor and activated both macrophages and DC, without leading to significant T cell proliferation. After 10 d on Ch, macrophages significantly down-regulated expression of pro-inflammatory markers, CD86 and MHCII. Production of pro-inflammatory cytokines, particularly TNF-alpha, decreased with time for cells cultured on Ch, while anti-inflammatory IL-10 and TGF-beta 1, significantly increased. Altogether, these results suggest an M2c polarisation. Also, macrophage matrix metalloproteinase activity was augmented and cell motility was stimulated by Ch. Conversely, DC significantly enhanced CD86 expression, reduced IL-10 secretion and increased TNF-alpha and IL-1 beta levels. Our findings indicate that cells with a common precursor may display different responses, when challenged by the same biomaterial. Moreover, they help to further comprehend macrophage/DC interactions with Ch and the balance between pro-and anti-inflammatory signals associated with implant biomaterials. We propose that an overall pro-inflammatory reaction may hide the expression of anti-inflammatory cytokines, likely relevant for tissue repair/regeneration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 18