Leptin effect on RANKL and OPG expression in MCM-E1 osteoblasts

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

M Lamghari (Autor) Outra; Tavares, L (Autor) Outra; Camboa, N (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP

Revista

Título: Journal of Cellular Biochemistry

Vol. 98

Páginas: 1123-1129

ISSN: 0730-2312

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 29 Citações

Scopus - 34 Citações

Outras Informações

ID Authenticus: P-004-J67

DOI: 10.1002/jcb.20853

Abstract (EN): Recent studies have suggested that leptin hormone may play a pivotal role on bone remodeling through a direct effect by modulating positively the OPG/RANKL balance. Here, we investigate the effect of leptin hormone on RANKL and OPG expression in MC3T3-E1 osteoblasts using RT-PCR and ELISA measurements. We have at first identified the expression of Ob-Rb and Ob-Ra leptin receptor isoforms in MC3T3-E1 and observed that these cells respond to mrleptin treatments. We then investigated the effect of mrleptin on RANKL and OPG expression. We show that mrleptin dose-dependently regulated the expression of RANKL mRNA with complete inhibition observed at concentrations higher than 12 ng/ml. This effect was confirmed with sRANKL protein measurements. However, the exposure of MC3T3-E1 to mrleptin had no effect on OPG mRNA. Taken together, these results suggest that leptin modulates positively OPG/RANKL balance by inhibiting the expression of RANKL gene.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7