Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

van Doorn, LJ (Autor) Outra; Figueiredo C (Autor) Outra; Rossau, R (Autor) Outra; Jannes, G (Autor) Outra; van Asbroeck, M (Autor) Outra; Sousa, JC (Autor) Outra; Carneiro F (Autor) FMUP; Quint, WGV (Autor) Outra

Revista

Título: Journal of Clinical Microbiology

Vol. 36

Páginas: 1271-1276

ISSN: 0095-1137

Editora: American Society for Microbiology

Indexação

ISI Web of Knowledge - 183 Citações

Scopus - 209 Citações

Outras Informações

ID Authenticus: P-001-80G

Abstract (EN): The present report describes an analysis of two virulence genes of Helicobacter pylori. Parts of the cagA gene, as well as parts from the signal (s) and middle (m) regions of the mosaic vacA gene, were amplified with biotin-labelled PCR primers and the products were subsequently analyzed by a single-step reverse hybridization line probe assay (LiPA). This assay comprises a strip containing multiple specific probes for the vacA s region (s1a, s1b, and s2 alleles), the, vac4 m region (m1 and m2 alleles), and the cagA gene. A total of 103 H. pylori-positive materials, including cultured isolates, gastric biopsy specimens, and surgical specimens from patients living in Portugal (n = 55) and The Netherlands (n = 48) were tested by the PCR-LiPA. cagA was detected in 83 and 73% of the Portuguese and Dutch patients, respectively. vacA typing results, as determined by reverse hybridization, were completely concordant with those of sequence analysis. Most Portuguese patients (72%) contained type s1b, whereas most Dutch patients (61%) contained type s1a (P < 0.001). The method is also very effective at detecting the presence of multiple genotypes in a single biopsy specimen. The prevalence of multiple strains in Portuguese patient samples was significantly higher (29%) than that in Dutch patient samples (8%) (P = 0.001). There was a significant association between the presence of ulcers or gastric carcinoma and the presence of vacA type s1 (s1a or s1b; P = 0.008) and cagA (P = 0.003) genes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6