Analysis of the bacterial community composition in acidic well water used for drinking in Guinea-Bissau, West Africa

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Machado, A (Autor) Outra; Adriano Bordalo (Autor) ICBAS

Revista

Título: Journal of Environmental Sciences

Vol. 26

Páginas: 1605-1614

ISSN: 1001-0742

Editora: IOS PRESS

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-009-QJD

DOI: 10.1016/j.jes.2014.05.027

Abstract (EN): Potable water is a resource out of reach for millions worldwide, and the available water maybe chemically and microbiologically compromised. This is particularly acute in Africa, where water-networks may be non-existent or restricted to a small fraction of the urban population, as in the case of Guinea-Bissau, West Africa. This study was carried out seasonally in Bolama (11 degrees N), where unprotected hand-dug wells with acidic water are the sole source of water for the population. We inspected the free-living bacterial community dynamics by automated rRNA intergenic spacer analyses, quantitative polymerase chain reaction and cloning approaches. The results revealed a clear seasonal shift in bacterial assemblage composition and microbial abundance within the same sampling site. Temperature, pH and turbidity, together with the infiltration and percolation of surface water, which takes place in the wet season, seemed to be the driving factors in the shaping and selection of the bacterial community and deterioration of water quality. Analysis of 16S rDNA sequences revealed several potential pathogenic bacteria and uncultured bacteria associated with water and sediments, corroborating the importance of a culture-independent approach in drinking water monitoring. (C) 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10