2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: Influence of endocannabinoid signalling in placental development

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Costa, MA (Autor) Outra; Keating, E (Autor) Outra; Fonseca, BM (Autor) Outra; Teixeira, NA (Autor) FFUP; Correia da Silva, G (Autor) FFUP

Revista

Título: Molecular and Cellular Endocrinology

Vol. 399

Páginas: 386-394

ISSN: 0303-7207

Editora: Elsevier

Indexação

ISI Web of Knowledge - 28 Citações

Scopus - 31 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-009-ZE8

DOI: 10.1016/j.mce.2014.09.005

Abstract (EN): A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The endocannabinoid system (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing I and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9