Detection of additional structural variation at the FES/FPS system and population data from S. Tome e Principe and North Portugal

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Pereira, L (Author) Other; Gusmao, L (Author) Other; Prata, MJ (Author) FCUP; Mota, P (Author) Other; Trovoada, MJ (Author) Other; Amorim, A (Author) FCUP

Journal

Title: International Journal of Legal Medicine

Vol. 112

Pages: 204-206

ISSN: 0937-9827

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-001-4QB

DOI: 10.1007/s004140050235

Abstract (EN): Comparative analysis of heteroduplex patterns on the STR FES/FPS sq stem led to the detection of a new non-consensus allele 10 in the African population of Sao Tome e Principe. Automated sequencing confirmed a T-->C substitution at position 177 (1(st)T of the 6(th) repeat) which was exclusively found in haplotypic combination with base A in the previously described polymorphic position 34, The new substitution was not detected in a sample from North Portugal. Sequence analysis revealed a triplet of inverted bases, from positions 101 to 103. relative to the sequence described in the GeneBank (Accession No. X06292), This work confirms the capacity of heteroduplex analysis in the detection of DNA structural microvariation and emphasises the complementary utility of manual system analysis and semi-automated techniques for a full characterisation of the genetic variability of STRs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3