pH Study and Partition of Riboflavin in an Ethyl Lactate-Based Aqueous Two-Phase System with Sodium Citrate

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Maria Eugénia Macedo (Author) FEUP; Pedro Velho (Author) FEUP; Inês Oliveira (Author) FEUP; Elena Gómez (Author) Other

Journal

Title: Journal of Chemical & Engineering Data

Vol. 67

Pages: 1985-1993

ISSN: 0021-9568

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 8 Citations

Scientific classification

CORDIS: Technological sciences

FOS: Engineering and technology

Associated Projects

LA LSRE-LCM - Financiamento Plurianual 2020-2023 para a Unidade LA LSRE-LCM Laboratório de Processos de Separação e Reacção - Laboratório de Catálise e Materiais

Other information

Authenticus ID: P-00W-75G

DOI: 10.1021/acs.jced.1c00909

Abstract (EN): Antioxidants neutralize reactive oxygen species in excess in animal bodies, ensuring a proper reduced state of the cells and avoiding the appearance of diseases. Since antioxidants are very reactive, they are heavily affected by changes in pH, which may influence partition and hamper solute quantification due to chemical structure and conformation modification. Natural antioxidants are present, for example, in fruit pomaces, and could be extracted for food supplementing purposes, contributing to a more circular economy. Aqueous Two-Phase Systems (ATPS) constitute a liquid-liquid extraction technique which has been applied with success in the recovery of bioproducts due to its sustainability and biocompatibility. Ethyl lactate and organic saltsbased ATPS are considered very eco-friendly because of their high biodegradability and low toxicity. In this work, the extraction of riboflavin (vitamin B2) was successfully performed in the ATPS {ethyl lactate (1) + sodium citrate (2) + water (3)} at 298.15 K and 0.1 MPa. This was accomplished after a careful study of the influence of pH in the UV-vis spectra and allowed to obtain a maximum partition coefficient (K) of 6.61 and a maximum extraction efficiency (%E) of 87.6% for the longest tie-line (TLL = 69.68) with antioxidant losses below 3%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9