Leptin effect on RANKL and OPG expression in MCM-E1 osteoblasts

• Type: Article in International Scientific Journal
• Date: 2006

Authors

M Lamghari (Author) Other; Tavares, L (Author) Other; Camboa, N (Author) Other; Mário Adolfo Barbosa (Author) FEUP

Journal

Title: Journal of Cellular Biochemistry

Vol. 98

Pages: 1123-1129

ISSN: 0730-2312

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-004-J67

DOI: 10.1002/jcb.20853

Abstract (EN): Recent studies have suggested that leptin hormone may play a pivotal role on bone remodeling through a direct effect by modulating positively the OPG/RANKL balance. Here, we investigate the effect of leptin hormone on RANKL and OPG expression in MC3T3-E1 osteoblasts using RT-PCR and ELISA measurements. We have at first identified the expression of Ob-Rb and Ob-Ra leptin receptor isoforms in MC3T3-E1 and observed that these cells respond to mrleptin treatments. We then investigated the effect of mrleptin on RANKL and OPG expression. We show that mrleptin dose-dependently regulated the expression of RANKL mRNA with complete inhibition observed at concentrations higher than 12 ng/ml. This effect was confirmed with sRANKL protein measurements. However, the exposure of MC3T3-E1 to mrleptin had no effect on OPG mRNA. Taken together, these results suggest that leptin modulates positively OPG/RANKL balance by inhibiting the expression of RANKL gene.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7