Instrumental Methods

Code:

Q3008

Acronym:

Q3008

Level:

300

Keywords
Classification	Keyword
OFICIAL	Chemistry

Instance: 2017/2018 - 1S

Active?	Yes
Responsible unit:	Department of Chemistry and Biochemistry
Course/CS Responsible:	Bachelor in Chemistry

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
L:Q	41	study plan from 2016/17	3	-	6	56	162

Teaching language

Portuguese

Objectives

Module 1 provides a systematic study of the modern techniques of gas chromatography, high-performance liquid chromatography, ionic chromatography and capillary electrophoresis. Emphasis will be placed on the theory, principle and application of these analytical separation techniques to real-world chemical analysis.

 

Module 2 deals with the use of different methods for determination of physical properties of materials (pure substances or mixtures). The theoretical foundation of each property will be presented, as well as the working principles of the techniques and respective instruments to be used.

 

Module 1

- describe and explain the general principles and theory of chromatography and capillary electrophoresis

- recognize the concept and instrumentation of gas chromatography, high-performance liquid chromatography and capillary electrophoresis.

 

 

Module 2

- perform the experimental determination of physical properties of materials

- recognize the importance and theoretical foundations of the various properties

- establish the relationships between different physical properties of materials and structural parameters of the studied compounds

 

Learning outcomes and competences

Module 1

-apply chromatographic and electrophoretic theories to improve separation selectivity and efficiency

-employ relevant chromatographic techniques to solve analytical chemistry problems

Module 2

- recognize the importance of physical properties in material characterization

- become familiar with instrumental methods that can be used to determine each property

- learn the foundations and characterization of different physical properties

- gain deeper knowledge of the particular methods to be used in the lab experiments – application, characteristics, limitations

- rationalize possible relations between the measured physical properties and structural parameters of the studied compounds

- develop the capacity to read/comprehend relevant scientific papers/book chapters

- develop the capacity to prepare and present oral presentations on the results obtained in the lab

Working method

Presencial

Program

Module 1

1 Chromatographic  methods

1.1 General theory of column chromatography

1.2 Optimizing chromatographic separations

1.3 Gas chromatography

1.4 High performance liquid chromatography

1.5 Ion chromatography

1.6 Other chromatographic techniques

 

2 Electrophoresis

2.1 Capillary electrophoresis

 

Module 2

3. Methods for characterization of materials, based on physical properties (refractometry, tensiometry, viscosimetry and heat capacity). Theoretical foundations and measurement techiques. Relationships between measured physical properties and structural parameters of the studied compounds.

3.1 Determination of molar refraction of organic liquids of a homologous series.

3.2 Determination of surface tension of liquids as a function of molecular parameters and temperature.

3.3 Determination of viscosity of organic liquids and its temperature variation.

3.4 Determination of the heat capacity of metals and solid or liquid compounds at one temperature (Drop-Cp method) and at different temperatures (differential scanning calorimetry).

Mandatory literature

Garland Carl W.; Experiments in physical chemistry. ISBN: 9780071263511
Skoog Douglas A.; Principles of instrumental analysis. ISBN: 0-03-002078-6
Daniel C. Harris, 8th Edition, W.H. Freeman and Co; Daniel C. Harris, 2007

Teaching methods and learning activities

Tutorial and lab classes.

 

- In the tutorials the concepts, principles and instrument description will be presented.

- In the lab classes

1. the students will perform the experimental determinations using the various techniques (group work)
2. they will present orally their experimental results, with subsequent discussion with the teacher and colleagues

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Participação presencial	100,00
Total:	100,00

Eligibility for exams

The students must attend a minimum of 3/4 of the total number of classes and carry out at least two thirds of the experimental work of the laboratory sessions to be admitted for evaluation.
If this condition does not hold the student fails to be admitted to evaluation.

Module 1

N1 = 0.30 AC1 + 0.70 EX1

AC1: Regular evaluation during laboratory sessions, evaluation and discussion of final report.
This evaluation is mandatory for all students.
Minimum grade: 9.5 (scale 0 to 20)

EX1: final exam

The final exam consists of a written exercise with theoretical questions in the form of multiple choice and numerical resolution of practical problems.
Minimum grade: 9.5 (scale 0 to 20)

 

Módulo 2

N2 = 0.40 AC2 + 0.60 PIA2

AC2: continuous evaluation of module 2 (40%)

PIA2: practical individual assessment of module 2 (60%)

Each component, AC2 and PIA2 has a minimum grade of 9.5 (scale 0 to 20)

Final grade

The final grade, FG, is calculated according to:

FG = 0.50 x N1 + 0.50 x N2

Calculation formula of final grade

 

Final grade

The final grade, FG, is calculated according to:

FG = 0.50 x N1 + 0.50 x N2

where

Module 1

N1 = 0.30 AC1 + 0.70 EX1

AC1: Regular evaluation during laboratory sessions, evaluation and discussion of final report.
This evaluation is mandatory for all students.
Minimum grade: 9.5 (scale 0 to 20)

EX1: final exam

The final exam consists of a written exercise with theoretical questions in the form of multiple choice and numerical resolution of practical problems.
Minimum grade: 9.5 (scale 0 to 20)

 

Módulo 2

N2 = 0.40 AC2 + 0.60 AO2

AC2: continuous evaluation of module 2 (40%)

AO2: Oral presentation and discussion of works done in module 2 (60%)

 

Special assessment (TE, DA, ...)

The special assessment shall be an oral examination.

Classification improvement

Distributed evaluation (AC1 and AC2) cannot be improved.

Module 1

The EX1 component can be improved in a retake final exam

 

Module 2

The PIA2 component can be improved in a complementary oral examination.