Code: | Q2013 | Acronym: | Q2013 | Level: | 200 |
Keywords | |
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Classification | Keyword |
OFICIAL | Chemistry |
Active? | Yes |
Web Page: | https://moodle.up.pt/course/view.php?id=2354 |
Responsible unit: | Department of Chemistry and Biochemistry |
Course/CS Responsible: | Bachelor in Chemistry |
Acronym | No. of Students | Study Plan | Curricular Years | Credits UCN | Credits ECTS | Contact hours | Total Time |
---|---|---|---|---|---|---|---|
L:BQ | 4 | Official Study Plan | 3 | - | 6 | 56 | 162 |
L:Q | 33 | study plan from 2016/17 | 2 | - | 6 | 56 | 162 |
3 |
After completing this course, the students should:
The students should have attended, and preferably completed successfully, the following courses Química I, Química II, Laboratório de Química I, Laboratório de Química II, Tratamento de Resultados em Química, Química Orgânica, Laboratório de Química Orgânica, Química Inorgânica e Laboratório de Química Inorgânica, or equivalent units.
Interaction between matter and light; absorption bands; relaxation mechanisms; atomic and molecular spectra. Spectrophotometers and how to measure UV/vis spectra. Lambert-Beer law and limitations. Energy levels of molecules; Franck-Condon principle; Intensity of absorption bands and selection rules. Energy diagrams of molecular orbitals to interpret electronic spectra; types of transitions in organic compounds; bathochromic and hypsochromic shift; hyperchromic and hypochromic shift; effect of multiple chromophores; effect of conjugation of double bonds; effect of substituents; application of Woodward-Fieser and Fieser-Kuhn rules; effect of solvent and pH; spectra of complexes of transition metals; types of transitions and intensity; how to obtain information about metal complexes from UV/vis spectra.
Introduction. Mechanism and absorption modes. Relation between bonding properties and absorption. Factors that determine the intensity and position of absorption bands. Fourier transform. Instrumentation. Sample preparation. Detection of functional groups. Structural analysis and identification, Applications and interpretation of spectra.
Introduction. Spectroscopic transitions and electromagnetic spectrum. Nuclear spin and nuclear magnetic moment. Mechanism of energy absorption (resonance). Population of nuclear spin states. Energy gap between nuclear spin states: interdependence of magnetic field and resonance frequency. Active nuclei in NMR. Chemical shift. Magnetic anisotropy. Magnetic equivalence. Effects of substituents on protection/deprotection. Spin-spin coupling. Coupling constant. Integration. Instrumentation and sample preparation. Deuterated solvents and solvent effect. 1st order spectra and 2nd order spectra. Typical chemical shifts in 1H NMR. 13C NMR spectra. Characteristic chemical shifts. Carbon-hydrogen coupling. Decoupled 13C spectra. Off-resonance decoupling. Factors that affect the chemical shift. DEPT sequences. Unidimensional (1D) and bidimensional (2D) spectra. Applications and interpretation of spectra.
Introduction. Ionization methods. Mass analysers and detectors. Instrumentation. Ion analysis. Isotopic abundance. Molecular ion, base ion and isotopic patterns. Electronic impact mass spectrometry and chemical ionization. Types of characteristic fragmentation and structural analysis. Molecular weight and molecular formula determination. Applications and interpretation of spectra.
Jablonski diagram; singlet and triplet states; Stokes shift; Half-life and quantum yield. Relation between fluorescence intensity and concentration of fluorophore. Fluorescence quenching and Stern-Volmer equation. Fluorescence anisotropy. Resonance energy transfer. Time-resolved fluorescence. Applications.
In the theoretical classes the main principles of each technique are presented and discussed, using experimental examples for a better understanding. In the problem solving classes, case studies are discussed relative to the interpretation of experimental data based on spectroscopic techniques. In addition, the students have three lab projects on UV/vis spectroscopy, FTIR and fluorescence spectroscopy.
designation | Weight (%) |
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Exame | 80,00 |
Trabalho laboratorial | 20,00 |
Total: | 100,00 |
designation | Time (hours) |
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Elaboração de relatório/dissertação/tese | 6,00 |
Frequência das aulas | 50,00 |
Trabalho laboratorial | 6,00 |
Total: | 62,00 |