Molecular Biotechnology

Code:

EQB4007

Acronym:

EQB4007

Level:

400

Keywords
Classification	Keyword
OFICIAL	Chemical and Biological Engineering

Instance: 2015/2016 - 2S

Active?	Yes
Responsible unit:	Department of Chemistry and Biochemistry
Course/CS Responsible:	Master in Food Science and Technology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:TCA	1	Official Study Plan	1	-	5	45	140

Teaching language

Portuguese

Objectives

The aims of the course are:

1. a) identify the basic principles underpinning the Recombinant DNA technology
2. b) associate to a molecular methodology its main application
3. c) demonstrate the ability to plan a molecular biotechnology application experience
4. d) Select the molecular methodology most appropriate for a particular purpose

e) Specify molecular biotechnology applications in food biotechnology

Learning outcomes and competences

By the end of the course the students should possess the tools and knowledge to select and use a given molecular methodology and should be able to plan an experiment involving the use of molecular biology towards biotech applications

Working method

Presencial

Program

The main goal of Molecular Biotechnology is to provide specific skills in the design of strategies to use correctly and efficiently the molecular techniques, in order to respond to the specific problems of food, environmental and pharmaceutical biotechnology:

- basic techniques of molecular biology (review) and its applications

-Recombinant DNA technology; cloning strategies in different organisms

- Production of recombinant proteins; particularities of different expression systems

- Metabolic engineering/synthetic biology

- Applications of Genomics, Proteomics and transcriptomics

- Molecular techniques in Food Biotechnology

– molecular microarrays, FISH identification, DGGE, RFLP, RAPD

- Approach to cases of practical application.

Mandatory literature

Old RW & Primrose SD; Principles of gene manipulation – an introduction to genetic engineering, Blackwell science. 5th Ed. , 1994
Brown TA ; Gene cloning: and introduction, Chapman&Hall. 3rd Ed , 1995
Videira A ; Engenharia Genética. Princípios e Aplicações, Lidel, 2011
Lima N, Mota M ; Biotecnologia, Lidel, 2004

Teaching methods and learning activities

Theoretical and practical classes

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation	Weight (%)
Teste	65,00
Trabalho escrito	35,00
Total:	100,00

Calculation formula of final grade

Evaluation by written exams (2) and by the presentation of a written report of na integrated project (report + oral presentation)