Instrumental Analysis

Code:

Q4000

Acronym:

Q4000

Keywords
Classification	Keyword
OFICIAL	Chemistry

Instance: 2015/2016 - 1S

Active?	Yes
Responsible unit:	Department of Chemistry and Biochemistry
Course/CS Responsible:	Master in Biochemistry

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BQ	34	Plano de Estudos do MBIOQ_2013-2014	1	-	6	56	162
M:TCA	3	Official Study Plan	1	-	6	56	162

Teaching language

Suitable for English-speaking students

Objectives

The major objective of this unit is to provide a thorough background in the physical and chemical principles that are particularly important to spectroscopic, electrochemical and chromatographic techniques. The second aim is to introduce a wide range of techniques that are useful in modern analytical chemistry (UV/Vis, photoluminescence, circular dichroism, emission and atomic absorption, potentiometry, voltammetry and chromatography). Additionally, students will develop the skills necessary to apply statistical tools that enable them to interpret analytical data. Finally, the last aim is to provide the laboratory skills that will give students confidence in their ability to obtain high quality analytical data.

 

Learning outcomes and competences

The course contents are consistent with the learning objectives, in the extent as knowledge of the composition and mode of operation of the different techniques, which govern the appearance of different analytical methods according with the specificity of chemical compounds, is provided for all topics the program

On the other hand, the knowledge of the analytical methods available to carry out chemical analysis allows students to identify the differences and similarities of these methods, as well the strategies adopted to conceal the physical and chemical interference of each. In laboratory classes will be developed technical skills fundamental to the implementation of some of the instrumental methods studied.

 

Working method

Presencial

Program

1 Spectroscopic techniques

1.1 UV/Vis Spectroscopy
1.2 Photoluminescence spectroscopy: (i) fluorescence and (ii) phosphorescence

1.3 Circular dichroism Spectroscopy

1.4 Applications

 

2 Atomic absorption and emission spectrometry

 

3 Electrochemical methods of analysis

3.1 Potentiometry

3.2 Chronoamperometry and chronocoulometry

3.3 Cyclic voltammetry and pulse voltammetric techniques

3.4 Applications

 

4 Chromatography of biomolecules

4.1 Ion exchange, size exclusion, hydrophobic interaction and reversed phase chromatography

4.2 Discussion of case studies

 

Mandatory literature

Skoog Douglas A.; Principles of instrumental analysis. ISBN: 0-03-002078-6
Harris Daniel C.; Quantitative chemical analysis. ISBN: 0-7167-1916-9
Miller James N. 1943-; Statistics and chemometrics for analytical chemistry. ISBN: 0-130-22888-5

Complementary Bibliography

Mikkelsen Susan R.; Bioanalytical chemistry. ISBN: 0-471-54447-7
Kellner R. 340; Analytical chemistry. ISBN: 3-527-30590-4

Teaching methods and learning activities

The teaching of instrumental analysis takes place through theoretical and practical classes and laboratory sessions. The theoretical and practical classes consist in detailed exposition of the content of the program, illustrated by study cases and problem solving. The audio-visual aids are used to support the presentations. In the laboratory sessions, the students carry out experimental works illustrative of some of the themes developed in theoretical classes, whose results and main conclusions will be presented and discussed orally.

 

The assessment will be continuous and will consider participation in discussion activities scheduled. Thus, the final grade of this course will have the following components: distributed evaluation (30%) and final exam (70%).

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	70,00
Participação presencial	15,00
Trabalho laboratorial	15,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Elaboração de relatório/dissertação/tese	0,00
Frequência das aulas	0,00
Trabalho laboratorial	0,00
Total:	0,00

Calculation formula of final grade

Final grade: 0.30 x Distributed Evaluation + 0.70 x Final Exam