Molecularly Imprinted Dispersive-Based Extraction Method for Melamine in Milk (MIDE HPLC DAD)

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Leandro Figueiredo (Author) FEUP; Lúcia Santos (Author) FEUP; Arminda Alves (Author) FEUP

Journal

Title: Journal Advances in Chemistry

Vol. 10 No. 6

Pages: 2775-2785

ISSN: 2321-807X

Indexing

ISI Web of Science

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

MEL-MIP - Polímero Impresso Molecularmente para Análise de Melamina em Produtos Alimentares

Other information

Authenticus ID: P-00S-CNG

Abstract (EN): An easy and selective molecularly imprinted dispersive extraction method (MIDE) coupled with HPLC-DAD was developed for melamine´s analysis in commercial milk samples. The molecularly imprinted polymer (MIP) was synthetized by suspension polymerisation with melamine as template, while methacrylic acid, ethylene glycol dimethacrylate and chloroform were used as monomer, cross-linker and porogenic solvent, respectively. The analytical methodology was validated presenting a limit of detection of 0.6 mg/kg. The recovery percentage in spiked milk samples ranged from 92.9% to 102.0% with an intermediate precision of 5.0%. The analysis of real samples with MIDE revealed melamine´s presence in 20% of the cases with a maximum concentration of 9.3 ± 0.3 mg/kg, which exceeds the maximum recommended level of 2.5 mg/kg.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11