Thiolated DAB dendrimer/ZnSe nanoparticles for C-reactive protein recognition in human serum

• Type: Article in International Scientific Journal
• Date: 2012

Authors

algarra, m (Author) Other; campos, bb (Author) Other; gomes, d (Author) Other; alonso, b (Author) Other; casado, cm (Author) Other; arrebola, mm (Author) Other; de los rios, mjd (Author) Other; herrera-gutierrez, me (Author) Other; seller-perez, g (Author) Other; da silva, jcge (Author) FCUP

Journal

Title: Talanta

Vol. 99 No. 7

Pages: 574-579

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 19 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-002-5F6

DOI: 10.1016/j.talanta.2012.06.037

Abstract (EN): A nanocomposite obtained by a thiol DAB-dendrimer (generation 5), coated with fluorescent ZnSe quantum dots, was successfully synthesized for the selective recognition of C-reactive protein. The procedure presented was carried out by a novel, cheap and non-toxic bottom up synthesis. The nanocomposite showed an excitation at 180 nm, with two emission bands at 411 and 465 nm, with a full-width at half-maximum of 336 nm. The Stokes shift was influenced by the presence of coating molecules and the intensity was dependent on pH due to the presence of a charge transfer process. The transmission electron microscopy images demonstrated that the spherical nanoparticles obtained displayed a regular shape of 30 nm size. The fluorescence intensity was markedly quenched by the presence of C-reactive protein, with a dynamic Stern-Volmer constant of 0.036 M-1. The quenching profile shows that about 51% of the ZnSe QDs are located in the external layer of the thiol dendrimer accessible to the quencher. The precision of the method obtained as relative standard deviation was 3.76% (4 mg L-1, n=3). This water soluble fluorescent nanocomposite showed a set of favorable properties to be used as a sensor for the C-reactive protein in serum samples, at concentrations of risk levels.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6