Identification of luciferyl adenylate and luciferyl coenzyme A synthesized by firefly luciferase

• Type: Article in International Scientific Journal
• Date: 2004

Authors

fraga, h (Author) Other; da silva, jcge (Author) FCUP; fontes, r (Author) FMUP

Journal

Title: ChemBioChem

Vol. 5

Pages: 110-115

ISSN: 1439-4227

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 37 Citations

Scopus - 38 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-BZQ

DOI: 10.1002/cbic.200300735

Abstract (EN): The firefly luciferase reaction intermediate luciferyl adenylate was detected by RP-HPLC analysis when the luciferase reaction was performed under a nitrogen atmosphere. Although this compound is always specified as an intermediate in the light-production reaction, this is the first report of its identification by HPLC in a luciferase assay medium. Under a low-oxygen atmosphere, luciferase can catalyze the synthesis of luciferyl coenzyme A from luciferin, ATP, and coenzyme A, but in air dehydroluciferyl coenzyme A was produced. The luciferase-catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl-coenzyme A synthetase.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6