Controlling the Surface Chemistry of Multiwalled Carbon Nanotubes for the Production of Highly Efficient and Stable Laccase-Based Biocatalysts

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Cláudia G. Silva (Author) FEUP; Ana P. M. Tavares (Author) FEUP; Goran Drazic (Author) Other; Adrián M. T. Silva (Author) FEUP; José M. Loureiro (Author) FEUP; Joaquim L. Faria (Author) FEUP

Journal

Title: ChemPlusChem

Vol. 79 No. 8

Pages: 1116-1122

ISSN: 2192-6506

Publisher: Wiley-Blackwell

Indexing

ISI Proceedings

ISI Web of Knowledge - 8 Citations

ISI Web of Science

Scopus - 17 Citations

Current Contents

COMPENDEX

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Technological sciences > Engineering > Chemical engineering

Associated Projects

LA 20 - PROJECTO ESTRATÉGICO - LA 20 - 2013-2014

Other information

Authenticus ID: P-009-RHD

DOI: 10.1002/cplu.201402054

Abstract (EN): Laccase was immobilized over multiwalled carbon nanotubes (MWCNTs) with diameters ranging from 10 to 100 nm and different surface chemical groups. Oxygen-containing groups were introduced selectively or removed by liquid-phase oxidation and thermal treatments. The effect of pH on the immobilization efficiency and catalytic activity of laccase has been evaluated. Pristine MWCNTs show excellent immobilization capacity (100%) and high enzyme activity, but low thermal stability (at 50 degrees C) owing to weak interaction (mostly hydrophobic) be-tween laccase and the support. Immobilization capacity and catalytic activity of laccase on the modified MWCNTs were related to the surface electric charge of both the support and the enzyme. MWCNTs oxidized with HNO3 and posteriorly heated at 400 degrees C, mostly presenting hydroxyl surface groups, provided the best compromise between laccase activity and thermal stability, which has been attributed to the formation of hydrogen bonds between the support and the enzyme.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7